{"id":13581,"date":"2017-08-16T04:02:22","date_gmt":"2017-08-16T04:02:22","guid":{"rendered":"http:\/\/union-sure.com\/?p=13581"},"modified":"2017-08-16T04:02:22","modified_gmt":"2017-08-16T04:02:22","slug":"high-glucose-induced-barrier-impairment-human-retinal-pigment-epithelium-ameliorated-treatment-goji-berry-extracts-modulation-camp-levels","status":"publish","type":"post","link":"https:\/\/www.union-sure.com\/high-glucose-induced-barrier-impairment-human-retinal-pigment-epithelium-ameliorated-treatment-goji-berry-extracts-modulation-camp-levels\/","title":{"rendered":"High glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels"},"content":{"rendered":"

\"\"<\/a><\/span><\/p>\n

High glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels<\/span><\/h1>\n

BarbaraPavan, AntonioCapuzzo, GiuseppeForlani<\/span><\/p>\n

Highlights<\/strong><\/span><\/p>\n

High glucose impairs tight junctions of human retinal pigment epithelial cells.<\/span>
\nHigh glucose elevates intracellular cAMP levels in HRPE cells.<\/span>
\nBarrier impairment correlates with cAMP accumulation in HRPE cells.<\/span>
\nHigh glucose directly activates the intracellular soluble adenylyl cyclase (sAC).<\/span>
\nGoji berry extract and taurine reverse high glucose\/sAC\/cAMP disrupting barrier.<\/span><\/p>\n

\n
\n

Abstract<\/strong><\/span><\/p>\n

\n

Human retinal pigment epithelium cells were used to investigate the mechanisms underlying blood-retinal barrier disruption under conditions of chronic hyperglycemia. The treatment with 25\u00a0mM glucose caused a rapid drop in the transepithelial electrical resistance (TEER), which was reversed by the addition of either a methanolic extract from Goji (Lycium barbarum<\/em> L.) berries or its main component, taurine. Intracellular cAMP levels increased concurrently with the high glucose-induced TEER decrease, and were correlated to an increased activity of the cytosolic isoform of the enzyme adenylyl cyclase. The treatment with plant extract or taurine restored control levels. Data are discussed in view of a possible prevention approach for diabetic retinopathy.<\/span><\/p>\n<\/div>\n<\/div>\n<\/div>\n

\n
\n

Keywords<\/strong><\/span><\/p>\n

HRPE \u00a0 \u00a0cells high glucose \u00a0 \u00a0plant extract \u00a0 \u00a0TEER \u00a0 \u00a0adenylyl cyclase<\/span><\/div>\n<\/div>\n<\/div>\n
<\/div>\n
<\/div>\n
*\u00a0This is a literature on the study of goji\u00a0berry and antioxidants .\u00a0You can click here <\/a>to read the full text.<\/em><\/span><\/div>\n","protected":false},"excerpt":{"rendered":"

High glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels BarbaraPavan, AntonioCapuzzo, GiuseppeForlani Highlights High glucose impairs tight junctions of human retinal pigment epithelial cells. High glucose elevates intracellular cAMP levels in HRPE cells. Barrier impairment correlates with cAMP accumulation in HRPE cells. …<\/p>\n

High glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels<\/span> Read More »<\/a><\/p>\n","protected":false},"author":1,"featured_media":13156,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"nf_dc_page":"","site-sidebar-layout":"default","site-content-layout":"default","ast-global-header-display":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[21],"tags":[],"yoast_head":"\nHigh glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels - NINGXIA UNION-SURE CO.,LTD<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.union-sure.com\/high-glucose-induced-barrier-impairment-human-retinal-pigment-epithelium-ameliorated-treatment-goji-berry-extracts-modulation-camp-levels\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"High glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels\" \/>\n<meta property=\"og:description\" content=\"High glucose-induced barrier impairment of human retinal pigment epithelium is ameliorated by treatment with Goji berry extracts through modulation of cAMP levels BarbaraPavan, AntonioCapuzzo, GiuseppeForlani Highlights High glucose impairs tight junctions of human retinal pigment epithelial cells. 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